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Description
Human METTL3 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against methyltransferase-like protein 3 (METTL3). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of methyltransferase-like protein 3 (METTL3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Methyltransferase-like protein 3 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Methyltransferase-like protein 3 (METTL3) is an enzyme encoded by the METTL3 gene. This gene encodes the 70 kDa subunit of MT-A, which is part of the N6-adenosine methyltransferase enzyme. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNA to form N6-methyladenosine (m6A). | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
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Great rug, especially for the price!
Size: 30" X 20", Color: Black
This rug was perfect for our small bathroom. It acts as a rug for the toilet and for the bathtub at the same time. So far it is holding up nicely and has fantastic grip on our lvp flooring and is so soft on my feet! 10/10 would buy again!
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Reviewed in the United States on May 13, 2026
★★★★★ 5
Nice little water/fertilizer.
Size: 1-Gallon
Nice little sprayer. It works well. its light weight and easy to use. Good value.
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Reviewed in the United States on May 24, 2026
★★★★★ 5
Great little sprayer
Size: 1-Gallon
This thing works really well. My only complaint is that you can't really get a tight cone on the mist setting. I wanted to be a little more precise, but it still got the job done. I would recommend this more for lawn treatments over bug spray (which is what I used it for), just because of the wide dispersal cone (especially indoors). However, it still works just fine, and gives a lot of coverage in a short amount of time. It does shoot a very precise stream out, but it's way too much liquid at once in my opinion. If you're wanting precision, you'll need to look elsewhere. As for the value, it's well worth it.
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Reviewed in the United States on May 25, 2026
★★★★★ 5
Chapin 1-gallon sprayer
Size: 1-Gallon, Size: 1-Gallon
★★★★★ Versatile Little Sprayer That’s Great Around the House, Garden & Garage.
This Chapin 1-gallon sprayer has turned out to be one of the handiest tools I own. I originally bought it for spraying organic bug treatment and neem oil mixtures on my plants and garden, but I’ve ended up using it for several different things.
The pump builds pressure quickly and the spray pattern adjusts easily from a fine mist to a stronger stream depending on what you’re doing. It sprays evenly and feels much more controlled than some of the cheaper sprayers I’ve tried in the past.
One thing I like is keeping separate sprayers for different jobs. I use this one mainly for garden and plant care, while I keep another one strictly for bug spray and outdoor treatments so chemicals don’t get mixed together.
It’s also useful outside the garden. You could easily use this for automotive detailing too — things like liquid waxes, degreasers, wheel cleaners, or spray-on protectants. The wand and hose feel durable, and the tank itself feels sturdy without being too heavy to carry around.
Simple, reliable, easy to clean, and useful for way more than just gardening. Definitely worth having around the house or garage.
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Reviewed in the United States on May 16, 2026
★★★★★ 4
Works well. Easy to carry.
Size: 1-Gallon 2-Value Pack
My 2-gallon sprayer’s nozzle cracked — leading to Spectracide dribbles along my path through the yard…not good!
Since a replacement nozzle cost as much as a new sprayer, so began the search for a new unit. I admit that lugging a full 2-gal sprayer around the yard, although it required no refilling, became quite tiring and blister-inducing; I then began reading reviews for 1-gal …
Horror stories about bad seals, leaks, no pressure, etc. had me dismayed. However, since Amazon’s return policy is easy, I decided on this 2-pack of 1-gal Chapin 22000. If needing my usual 1.5-2 gallons of weed killer, I could always fill BOTH ahead of time and save myself some blisters and backache!
Never having used pressurized sprayers WITHOUT pressure relief valves, I was slightly hesitant to buy these, though…well, I guess I’ll just have to be extremely careful to not get the poisonous spray on myself when removing the pump! ☠️
Sprayers arrived in perfect, brand new condition; all parts/pieces were received. Assembly was super easy, even WITHOUT referring to instruction manual — used channel locks to tighten the hose, as pliers could not be located and hands are not very strong. Tested/practiced with water. Got a feel for how many pumps were necessary and adjusted the spray tip to my preference.
My last sprayer required opening the PRV and then squeezing sprayer to empty hose/release remaining pressure in order to be able to unscrew pump handle. The directions for THESE say to simply unscrew the handle a bit until you can hear the built-up pressure vent out. I did just that — no explosive spray to the face and the pump handle unscrewed easily. WHEW!
So, today was the sprayer’s maiden voyage around the yard.
I followed the directions to pump the handle until resistance is felt (maybe 8x?). WOW — so much easier to carry! Pressure is great — liquid shot out about 15’! Although the “feel” of the sprayer trigger is cheap and small (even for female hands), it was quite responsive and allowed for a steady stream with no leaks/dribbles.
Happy with this purchase and hope I can get at least a few years out of them. 🤞🏼
Also, after I’d placed my order, I just happened to be in WM’s lawn/garden section…
These are sold there, as well, for a higher price. They seem to be very popular, too, as nearly all were gone from the shelf.
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Reviewed in the United States on April 1, 2026
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