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Description
Rat TGF-b2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 1000pg/mL). Then dilute to the following concentrations: 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.625pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 1000pg/mL standard working solution into the first EP tube and mix thoroughly to make a 500pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a Transforming Growth Factor Beta 2 (TGF-b2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Transforming Growth Factor Beta 2 (TGF-b2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Transforming Growth Factor Beta 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Transforming growth factor β2 (TGF-β2), also known as glioblastoma-derived T-cell suppressor factor (G-TSF or BSC-1), is a secreted protein called a cytokine. It performs many cellular functions and plays a crucial role in embryonic development. It is an extracellular glycosylated protein and is known to inhibit the action of interleukin-dependent T-cell tumors. It regulates various processes, including angiogenesis and cardiac development. Its immune-regulating effects include: reducing inflammation: Through regulatory T cells, it helps balance the immune system and reduce inflammatory substances; reducing allergic reactions: It reduces the level of allergies caused by IgE (immunoglobulin E), reducing hypersensitivity; improving food tolerance: It helps build tolerance and mitigates the risk of allergies to foreign proteins in food; and protecting against viral and bacterial invasion: It promotes IgA (immunoglobulin A) secretion, which helps the intestinal mucosa develop and resists foreign invasion; and protecting against influenza A virus (H1N1) infection, reducing the severity and duration of the disease. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 15.62-1000 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.3 ★★★★★
Based on 2012 reviews
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Product Reviews
★★★★★ 5
helped treat my sore neck
Color: White-cooling Basic, Size: Camping Pillow(Pack of 1)
This pillow — after dialing in the right amount of shredded foam — has greatly helped my sore neck. Initially, I had to remove some foam. Then I gradually added foam until it reached optimum comfort and support.
It is also CPAP-friendly — especially when I position my head near the pillow's edge. (I am a left-side sleeper.)
I like the travel size because I use a bulky hugging pillow to ease stress on my shoulder and elbow. That makes my side of the bed feel cramped if using a larger head pillow. A smaller pillow is easy to flip end-to-end in the middle of the night.
I have spent lots of money on pillows over the years, but this is the best I've ever had for relieving [1] neck pain and [2] CPAP mask seal problems. Very comfortable, supportive, and affordable.
I did not notice any unpleasant smell — and I am usually sensitive to that kind of thing.
Would highly recommend.
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Reviewed in the United States on May 7, 2026
★★★★★ 5
Love them!
Color: White-cooling Basic, Size: Queen(Pack of 2)
Love them! After 10 years with our MyPillows, they finally lost their fluff. The reviews I read of the MyPillows of today were not good; they didn’t have as much filling as they used to. These pillows remind me of how fluffy our new MyPillows were 10 years ago!! You sink right into them and can mush them however and they stay. These are way cheaper than the Coop and it’s the same concept; add or remove the filling as needed. The cooling side is a huge plus too! You won’t be disappointed with these pillows!
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Reviewed in the United States on May 22, 2026
★★★★★ 5
super smooth, silky, stays comfortable feeling. DO NOT DRY or wash with heat
Color: Blue, Size: Queen (U.S. Standard), Set name: Stretchy and Soft, Color: Blue, Size: Queen (U.S. Standard), Set name: Stretchy and Soft
I'm impressed. I have used cooling pillow cases before, and found them effective, but the cooling material have always been on just one side. These are 100% cooling material. The material is super smooth and silky feeling. It is great feeling against hair and skin (even better than my old polyester satin I was using before cooling pillowcases). The material is cool, and does not seem to get hot hot. The pillow cases are super stretchy with a small hidden zipper. They grab onto the pillow and dont loosen up. Makes my pillow look neat and clean, not sloppy from loose material. I can even have two pillows stacked and somehow despite being a smooth material, the pillows dont just slide off each other.
The cooling aspect works by not trapping heat, not allowing heat to get to the pillow and hold it there. So yeh, the pillow case will feel warm eventually but if you have a fan, or AC, or flip the pillow, then it is is truly cool. Otherwise in my experience when I use a pillow without this, the pillow just gets hot, stays hot, and even with flipping it feels hot.. because the pillow itself has trapped the heat for remainder of night. Yuck.
One thing to know and this is very important-- as I learned the hard way-- DO NOT DRY IN DRYER. DO NOT WASH IN HOT WATER. Heat will destroy the material ability to feel and stay cool. Do you want a regular heat trapping pillow case? Then go ahead and dry these in the dryer a few times and you will have a regular pillow case. HOw do I know? I bought these to replace another set (different brand, same idea, same instructions that I failed to remember). I always dried them in the dryer, and one night after having gloriously cool pillows, I noticed that my pillow was HOT night after night. It was then when looking for another set of pillow cases that I learned that they cant be exposed to heat like that. SO PLEASE, just AIR DRY.
And so, after I got these and put them on my pillows, I had a much better nights rest. I remember thinking that I wasnt waking to rearrange my pillows. They were just comfortable all night long. Sure, I'd love an even colder pillow, but our room is on the warm side. But at least I wasnt noting the hot hot pillows anymore.
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Reviewed in the United States on June 6, 2021
★★★★★ 5
A must try for hot sleepers!
Color: Blue, Size: Queen (U.S. Standard), Set name: Silky, Color: Blue, Size: Queen (U.S. Standard), Set name: Silky
If you are a hot sleeper these pillowcases are for you! Not only are they silky and beautiful, but they are cool to the touch!The Arc-Chill fabric absorbs heat and instantly cools your skin! If you are prone to insomnia, night sweats, give these a try! They come in a 2 pack and have a few different colors to try out. Highly recommend.
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Reviewed in the United States on May 27, 2026
★★★★★ 5
Freezing !
Color: Gray, Size: Queen (U.S. Standard), Set name: Stretchy and Soft
### Product Review: Avolare 2 Pack Cooling Pillow Cases
#### Overview
The Avolare 2 Pack Cooling Pillow Cases are designed to provide a cooler and more comfortable sleeping experience, especially for hot sleepers. Made with Arc-Chill fabric, these pillowcases promise to offer a cooling effect, protect hair and skin, and add a touch of luxury with their soft and silky texture. The pillowcases come in a standard gray color, suitable for standard and queen-size pillows.
#### Material and Design
**Rating: ★★★★★**
The Avolare Cooling Pillow Cases are crafted from Arc-Chill fabric, known for its high Q-max value (>0.5), indicating excellent cooling properties. The material feels incredibly soft and silky to the touch, providing a luxurious feel that enhances sleep quality. The gray color is neutral and versatile, fitting seamlessly with various bedding styles.
The design includes a hidden zipper, ensuring that the pillowcase stays securely on the pillow while maintaining a smooth and sleek appearance. The zipper is discreet and doesn’t interfere with comfort, which is a thoughtful addition to the overall design.
#### Cooling Effect
**Rating: ★★★★★**
One of the standout features of these pillowcases is their cooling effect. The Arc-Chill fabric effectively wicks away heat and moisture, making them ideal for hot sleepers or those living in warmer climates. Users have reported a noticeable difference in temperature, which contributes to a more restful and comfortable night’s sleep. This cooling property is particularly beneficial during the summer months or for individuals who tend to overheat while sleeping.
#### Hair and Skin Benefits
**Rating: ★★★★☆**
In addition to their cooling properties, the Avolare pillowcases are also beneficial for hair and skin. The smooth and silky texture reduces friction, helping to prevent hair breakage and skin irritation. This can be particularly advantageous for individuals with sensitive skin or those prone to hair damage. While the pillowcases are not a cure-all for skin and hair issues, they do contribute positively by creating a gentler surface.
#### Durability and Care
**Rating: ★★★★☆**
The Avolare Cooling Pillow Cases are easy to care for, maintaining their cooling properties and softness even after multiple washes. They are machine washable, which adds to their convenience. However, it is recommended to follow the care instructions carefully to ensure the longevity of the cooling effect and fabric quality.
#### Versatility
**Rating: ★★★★☆**
These pillowcases are designed to fit both standard and queen-size pillows, adding to their versatility. They are perfect for use in any bedroom setting, from master bedrooms to guest rooms, and can be paired with various pillow types and fillings.
#### Pros and Cons
**Pros:**
- Excellent cooling properties ideal for hot sleepers.
- Soft and silky texture is gentle on hair and skin.
- Hidden zipper design for a secure fit.
- Machine washable and easy to care for.
- Versatile fit for standard and queen-size pillows.
**Cons:**
- Cooling effect may diminish slightly with frequent washing.
- Limited color options (only available in gray).
#### Final Verdict
**Overall Rating: ★★★★☆**
The Avolare 2 Pack Cooling Pillow Cases are an excellent choice for anyone looking to enhance their sleeping experience, particularly hot sleepers. The cooling properties of the Arc-Chill fabric, combined with the soft and silky texture, provide a comfortable and luxurious feel. The hidden zipper design adds practicality without sacrificing aesthetics.
While there are minor drawbacks, such as potential diminishing of the cooling effect with frequent washing and limited color options, the overall benefits make these pillowcases a worthwhile investment. If you’re seeking to improve your sleep quality and enjoy a cooler, more comfortable night’s rest, the Avolare Cooling Pillow Cases are definitely worth considering.
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Reviewed in the United States on June 1, 2024