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Description
Human TREM2 ELISA KitProduct Specification protein TREM2 Usage 1. Self brought consumables: 1 , plate reader (capable of measuring 450 nm Absorbance at) 2 , multi channel pipette or automatic plate washer 3 , incubator, cryogenic centrifuge 4 Adjustable pipette gun and gun tip 5 Deionized water 2. Reagent preparation: Standard Diluent Ready to use; Before use, equilibrate to room temperature; 2 8 Save. Standard Diluent Used to dilute standard. 1Assay Buffer Before use,
Product Specification
| protein | TREM2 | ||||||||||||||||||||||||||||||||||||||||
| Usage |
1. Self-brought consumables: 1 , plate reader (capable of measuring 450 nm Absorbance at) 2 , multi-channel pipette or automatic plate washer 3 , incubator, cryogenic centrifuge 4 Adjustable pipette gun and gun tip 5 Deionized water 2. Reagent preparation: Standard Diluent ： Ready-to-use; Before use, equilibrate to room temperature; 2-8℃ Save. Standard Diluent Used to dilute standard. 1×Assay Buffer ： Before use, equilibrate to room temperature and press with deionized water 1 ： 10 Proportional dilution of Assay Buffer (10×) get 1×Assay Buffer , mix gently to avoid foaming. 2-8℃ Preservation, this solution can be stable 30 Heaven. 1×Assay Buffer For diluting serum and plasma samples, HRP-conjugated Human TREM2 Detect Antibody (100×) 。 Human TREM2 Standard ： The label superscript added to the standard focuses on suspension volume reconstitution in deionized water Human TREM2 Standard get 20 ng/mL Standards. Before performing dilution, hold the standard at least 15 min And gently shake, after reconstitution Standard Available at -20℃ Save 1 Months, redissolved and used 1 Discard after times. 1×HRP-conjugated Human TREM2 Detect Antibody ： Mix thoroughly before dilution. Depending on the amount required for standard and sample, use in clean plastic tubes 1×Assay Buffer Right HRP-conjugated Human TREM2 Detect Antibody (100×) Conduct 1:100 Dilution. 1×HRP-conjugated Human TREM2 Detect Antibody Should be after dilution 30 min Internal use. HRP Substrate (TMB) ： Ready-to-use; Before use, equilibrate to room temperature; 2-8℃ Store in the dark from light. Stop Solution ： Ready-to-use; Before use, equilibrate to room temperature; 2-8℃ Save. 1×Wash Buffer: Equilibrate to room temperature before use, Wash Buffer (20×) Performed with deionized water 1:20 Dilution to obtain 1×Wash Buffer 。 Mix gently to avoid blistering, store at room temperature, this solution is stable 30 Heaven. Standard curve settings: As shown in the table below, use Standard Diluent Will 20 ng/mL The standard was diluted to 10 、 5 、 2.5 、 1.25 、 0.63 、 0.31 、 0.16 And 0 ng/mL Of Human TREM2 Standards.
Note: Each experiment requires a new formulation of standards. 3. Sample preparation: 1 Cell culture supernatant: centrifuge to remove particles, immediately measure or dispense -20℃ Store and avoid repeated freezing and thawing. 2 Serum: Use a serum separation tube and allow the sample to coagulate at room temperature 30 min , then 1,000 g Centrifugation 15 min 。 Take the upper layer serum for immediate determination or dispensing -20℃ Store and avoid repeated freezing and thawing. 3 Plasma: use EDTA , heparin or citrate as an anticoagulant. After collection 30 min Inner 1,000 g Centrifugation 15 min 。 Immediate determination or dispensing -20℃ Store and avoid repeated freezing and thawing. CAUTION: Do not use specimens with severe hemolysis or lipidemia. If the sample is to be in 24 h Used within, you can store it in 2 To 8℃ , avoid repeated freezing and thawing. Frozen samples should be slowly warmed to room temperature and gently mixed prior to determination. 4. Experimental steps: 1 Remove the excess slats from the plate frame, place them back in the aluminum foil bag containing the desiccant pack, and reseal them. 2 The liquid in each well was removed and washed. Add per well using a multi-channel pipette or automatic washer 300 μL 1×Wash buffer Let stand and soak 30 s , performing washing. Complete removal of the liquid in each step is essential to obtain good performance. After the last wash, the plate was inverted and blotted dry with a clean paper towel to remove any remaining 1×Wash Buffer 。 Use the plate immediately after the plate wash is completed and do not allow the plate to dry. 3 , Add standard: add standard well 100 μL Diluted standards. 4 Add sample: serum / Plasma: sample well addition 80 μL 1×Assay Buffer And 20 μL Pre-diluted samples; Cell culture supernatant: sample well addition 100 μL Cell culture supernatant (See sample preparation for details). 5 , add detection resistance, add each well 50 μL 1×HRP-conjugated Human TREM2 Detect Antibody , guarantee step 3 、 4 、 5 Add samples continuously without interruption. The sample loading process is 15 min Within completion. Plate was coated and incubated at room temperature 2 h 。 6 Repeat steps 2 Washing in 6 Times. 7 , adding per well 100 μL HRP Substrate (TMB) 。 The plate was coated and incubated at room temperature protected from light 5-30 min 。 8 , adding per well 100 μL Stop Solution ， Stop Solution Should be in accordance with the TMB The same order is added to the plate. The color in the hole should change from blue to yellow. If the color in the hole is green or the color changes unevenly, tap the plate lightly to ensure thorough mixing. 9 , in 30 min Within, measure each well 450 nm The absorbance value at. Note: 5-30 min The color development time is within the empirical range. For each specific experiment, the approximate color development time can be determined according to the following conditions. （ 1 ) Visual observation: standard song S5 The holes are light blue, Blank When the hole has no obvious blue color, it can be terminated; （ 2 ) Instrument judgment: 630 nm At left and right wavelengths, standard curve S1 Porous OD Value reaches 0.5 - 0.7 、 S5 Porous OD Value reaches 0.05 - 0.08 、 Blank Porous OD Value less than 0.05 It can be terminated. 5. Result calculation: 1 Calculate the multiwell average of each standard and sample OD Value, and subtract the zero concentration ( Std.8 ) average OD Value. 2 , Drawing of standard curve: take the standard concentration as x Axis, the average absorbance of each standard is y Axis, plot the standard curve. You can use mapping software to create standard curves. Note: If the sample is diluted, the concentration read from the standard curve must be multiplied by the dilution factor. 6. Results display: Typical standard curve ( R2≥0.99 ） Figure1. 96 Well plate analytical Human TREM2 Standard curve. The data and curves are for reference only, and experimenters need to establish standard curves according to their own experiments. |
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| Sensitivity | 0.16 ng/mL | ||||||||||||||||||||||||||||||||||||||||
| Species Reactivity | Human | ||||||||||||||||||||||||||||||||||||||||
| Source | Human | ||||||||||||||||||||||||||||||||||||||||
| Synonym | Human TREM-2 ELISA Kit | ||||||||||||||||||||||||||||||||||||||||
| Detection Type | Sandwich method | ||||||||||||||||||||||||||||||||||||||||
| Description | Myeloid cell trigger receptor 2 (TREM2) is a single Ig domain receptor that is expressed in macrophages and dendritic cells, but not in granulocytes or monocytes. It is most abundantly expressed in the basal ganglia, corpus callosum, medulla oblongata and spinal cord, while microglia in the central nervous system are the most dominant cell type producing TREM2. TREM2 plays a role in chronic inflammation and can stimulate the production of constituent rather than inflammatory chemokines and cytokines. TREM2 forms a receptor signaling complex with TYROBP to activate the immune response of macrophages and dendritic cells. It is also associated with the adaptor protein DAP12 to transmit activation signals, which play a role in innate and adaptive immune responses. TREM2 Signaling is also an important way to promote colonic wound healing. TREM2 mutations increase the risk of neurodegenerative diseases such as Alzheimer's disease, amyotrophic lateral sclerosis, and Parkinson's disease. The Human Myeloid Cell Trigger Receptor 2 ELISA Quantification Kit employs a double antibody sandwich method to quantify human myeloid cell trigger receptor 2 in a sample. The plate is coated with an antibody specific to human myeloid cell trigger receptor 2, and the sample or standard and the specific detection antibody labeled with HRP are added to the wells of the plate. The human myeloid cell trigger receptor 2 binds to the solid phase antibody and the detection antibody coated on the plate. After washing, a chromogenic substrate (TMB) was added, and the TMB became blue under HRP catalysis and turned yellow after addition of the stop solution. The OD value was measured with a microplate reader at 450 nm wavelength, and the concentration of human myeloid cell trigger receptor 2 was directly proportional to the OD450 value. |
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| Composition |
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| General Notes | 1. If Standard Diluent and Assay Buffer (10 ×) turn yellow in color and have a small amount of precipitation, it is because the reagent contains serum. Just centrifuge to remove the precipitation, which will not affect normal use. 2. Do not mix components with different batch numbers and different manufacturers; Failure to do so may lead to abnormal results. 3. Change the tip frequently to avoid cross-contamination between the components. 4. To ensure accurate results, the plate must be properly adhered during the incubation step. 5. Stop Solution is corrosive to a certain extent, so please take protective measures when operating. |
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| Instructions | Serum (plasma), cell culture supernatant | ||||||||||||||||||||||||||||||||||||||||
| Storage Temp. | 2-8 ℃, protected from light, valid for 24 months. | ||||||||||||||||||||||||||||||||||||||||
| Test Range | 0.16 ng/mL-10 ng/mL |
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4.7 ★★★★★
Based on 1893 reviews
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Product Reviews
★★★★★ 5
Best toy, the end
Style: Sport, Size: 12in, Style: Sport, Size: 12in
Combined with the Max Glow ball, this is the best dog toy available on modern planet Earth.
I have a burly 92lb. pit bull mix with highly developed prey drive, and she will chase the glow ball for hours if I let her.
The tiny sport launcher (Sport 12M) is the best, because you can still throw the ball really far, but you can also slam the ball down a few feet away from yourself so it bounces up in the air and your dog has to jump to catch it, or you can throw it up really high so it bounces 15' away or so and your dog can jump to catch it. We sometimes have to play fetch in a pretty small area, so it's nice to have the small launcher for these games. The larger launchers aren't as fun in small areas. You can play the same games but it's trickier to throw the ball correctly.
Also, the small launcher is a lot easier to carry. The small launcher fits in my dog-walk-stuff backpack (with the ball in the launcher so I don't have to dig for it later), but the big ones don't easily. The launcher also has a hole in the end so you can hang it from your bag or whatever with a carabiner. The big ones are too long for that in most cases, and swing back and forth.
Also-also my dog loves to ask for the launcher, then when I give it to her she plays keep-away and it's adorable. The big launcher I have is harder for her to balance in her mouth, so she just lays down and chews on it until it gets taken away. She's chewed on all of the four Chuck Its we've owned, but she's only broken one (a big one), but that's because she got ahold of it when no one was home. The little one in my bag is totally chewed up but still works great. I think they're sturdier than the big ones. Except for the launcher she chewed into pieces, which was our fault, the only reason we've had to replace anything was because we lost it. Usually we keep the launcher and ball in the backpack so she can't get to them and so they don't get lost, so we rarely have to replace them. There is just not a better value in toys, and the startup price is really low.
Once again I really recommend the glow ball! It doesn't get soggy no matter how wet the grass or the dog mouth is. It is fun to chase because it is slippery and it collapses when it's squeezed, so it pops out of the dog's mouth and flies away if they bite it at the wrong angle; it's really bouncy, and stays bouncy because it can't be popped; it whistles so you can usually hear it even if you can't see it; and it really glows enough for nighttime catch, even if you only have your cellphone flashlight to charge it up!
UPDATE:
I should have said, we have the 12M launcher, which means the handle is 12 inches long and it uses a medium-sized ball. The medium ball is 2.5 inches in diameter, so the launcher will fit regular tennis balls, too! But my dog won't play with regular tennis balls anymore because they aren't anywhere near as fun as the glow balls, and I think she doesn't like having gross sloshy muddy tennis balls in her mouth.
I lost my short launcher awhile back and could only get a long one locally to replace it, which reminded me that you have to lean over a lot further to use the short launcher. So if you have trouble leaning over to the point that your hand is a foot above the ground, this may be hard for you to use.
I wish Amazon would let us choose colors, because my old one was green, and the big one is orange, both of which are bright enough to see in the grass from far away, even at night.
Still, best toy!
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Reviewed in the United States on May 19, 2017
★★★★★ 5
Highly recommend.
Style: Sport, Size: 12in
Love this. Dog sitting for a dog that loves to chase balls but my shoulder doesn’t like throwing them. Got this yesterday and it’s perfect. Real arm/shoulder saver.
Seems sturdy and I was concerned that a standard tennis ball would not work. We tend to lose balls when walking because this lab insists on carrying the ball in its mouth. They work.
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Reviewed in the United States on March 22, 2026
★★★★★ 5
These are awesome and I couldn't play ball with my Dobermans without it!
Style: Sport, Size: 12in
I LOVE Chuckit ball launchers. We've been using for a around 17 years and we've only had to replace one. We have 2 active, energetic Dobermans and I throw like a girl and there is now way I can throw a ball very far. With the Chuckit, I can throw more than 80 feet! My dogs need a lot of exercise and using this ball thrower allows me to make that happen. Bonus is I dont have to pick up a snobbery ball :)
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Reviewed in the United States on April 13, 2026
★★★★★ 4
Not a long shot
Style: Sport, Size: 12in
This item is much smaller than I thought it would be .
The ball is full sized . But the thrower is very small.
Works okay. But does not throw the ball very far . It’s okay for me . My dogs are small. My yard is too .
But , this is not going to throw balls really far . Like the long ones do
Thsnks
Eddie
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Reviewed in the United States on March 26, 2026
★★★★★ 5
Perfect addition
Style: Sport, Size: 12in
This is item that I needed but didnt know it. Its been great for saving my sholder anad my dog loved the longer chase to get the ball. Easy to use and lots of fun
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Reviewed in the United States on May 18, 2026