SKU: 9818138884

Human TNF-α High Sensitivity ELISA Kit

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Description

Human TNF-α High Sensitivity ELISA KitProduct Specification Synonym APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte derived; TNFA; TNF A; TNFalpha; TNF alpha; TNF alphacachectin; TNFATNF, macrophage derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor alpha Description Testing Principle This

Product Specification

Synonym APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
Description

Testing Principle:
This kit uses double antibody sandwich ELISA.Specific anti-human TNF-α Capture Antibody is precoated on a high affinity conjugate plate.  Standard substance, sample to be tested and biotin labeled detection antibody were added successively into the plate well of the enzyme label. After fully shaking and mixing, the samples were placed at room temperature for a 2-hour incubation process. The TNF-α in the samples was bound to the solid phase antibody and detection antibody.  After washing thoroughly to remove free and unbound ingredients, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is added.  After washing, signal enhancer was added for incubation. After washing and removing unbound substances, SA-HRP was added again.  After washing again, TMB color substrate was added and incubated in dark at room temperature for color development.  The depth of color reaction was positively correlated with the concentration of TNF-α in the sample.  The termination solution was added to terminate the reaction, and the absorbance value was measured at 450 nm detection wavelength (correction wavelength 570-630 nm) using a microplate analyzer.  


Detection type:Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity:100 μl

Kit composition:pre-coated 96-well plate,Standards,Standard, TNF-α Detector Antibody, Standard Diluent, Detector Buffer, Signal Enhancer, Signal Enhancer Diluent, TMB Color Substrate, Washing Solution, Termination Solution, SA-HRP, Sealing Plate Mask and Manual  

Sensitivity:0.16 pg/mL

Detection range:1.56-100 pg/mL

Recovery rate:100-108%

Storage:2-8℃

The standard curve

 

Background:

Tumor necrosis factor α(TNF-α), also known as cachexin and TNFSF1A, is an adipokine that is involved in systemic inflammation and is one of the cytokines that stimulate acute phase responses. It is produced mainly by activated macrophages, but can also be secreted by other types of cells, such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It has a variety of regulatory functions in the immune response, but also can be used as a potential pyrogen. In response to stimuli (infectious agents or tissue damage), TNF-α circulates throughout the body, activates neutrophils, alters the properties of vascular endothelial cells, regulates metabolic activity in other tissues, and exhibits tumor-killing activity by inducing local coagulation. TNF-α plays an important role in the pathogenesis of inflammation in joint tissues and other tissues. Recently, more and more information indicates that TNF-α is also involved in the pathogenesis of AIDS. The determination of TNF-α is also useful for transplantation studies.  

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SKU: 9818138884

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4.2 ★★★★★
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Annie Sameuchay
New York, US
★★★★★ 5
This company makes great toys for pitts!!
Color: Blue/Medium, Size: Blue, Color: Blue/Medium, Size: Blue
Our pitt LOVES this toy. The rubber is reeeaally tough. It’s well designed because it’s a slippery surface. You can tell from the video there’s a lot of squeaking from him trying to get a good grip. He found a pretty good spot to hook his upper teeth into the crevasse, but his bottom teeth kept slipping. As a side note, I wouldn’t leave any toy home alone with him that’s about 3 inches or smaller because dogs can choke on it, but that doesn’t make this any toy or company any less amazing. For toys I would leave home with a power chewer, take a look at that 4inch red ball made by this same company. He hasn’t made a dent in that thing and you can put treats in it. :)
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 5, 2025
A
Verified Purchase
Amazon Customer
Chelsea, US
★★★★★ 1
No for aggressive chewer waste of money
Color: Blue/Medium, Size: Blue, Color: Blue/Medium, Size: Blue
Within 10 minutes she destroyed it
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Reviewed in the United States on June 1, 2026
M
Verified Purchase
Maria M.
Battle Creek, US
★★★★★ 5
Favorite toy!
Color: Orange/Large, Size: Orange
My dog lives these!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 23, 2026
S
Verified Purchase
Shorty & Lily's Mom
Grantham, US
★★★★★ 5
Takes a beating - looks like new!
Color: Blue/Medium, Size: Blue
EDITED AGAIN, 12/28/22: My boy still has his original blue one of these and the yellow one from way back. He's received a couple more over the years which he's managed to sneak out of the house and lose - evidently neighbor dogs or perhaps foxes, etc love them, too, and carry them off?! At any rate, he'd managed to get himself back to his original two, so I bought him a late Christmas present of a new yellow bone. He just opened the package (with help, of course - he's smart, but lacks the necessary opposing thumbs to do it on his own) and he was ecstatic! He reached into the mailing envelope, grabbed it, gave it a couple of triumphant tosses then ran for his bed, pulled a blanket in, and has been chewing, fighting with, burying in the blanket and "discovering" it for about a half hour now! I don't know what the attraction is, but he still loves these things and they're still lasting! The only other toys that have lasted more than a day are Nylabones (because he won't even look at them, let alone touch them) and a set of interlocked skinny rubber rings that he's not at all interested in, but if I ask about them, he'll move them around the room for a couple of days to make me feel better! LOL EDITED TO ADD 2022: Here I am, back 5 1/2 years later to say my dog STILL has this bone and makes use of it a couple of times a week. And he still loves it and sleeps with it. He has gone through a couple of the smaller version (yellow instead of blue) over this time frame because those get constant use. But that’s amazing in itself!! These are still the only toy I would call indestructible. Great toy! I have a dachshund who destroys toys in record time. I can hear the amused chuckles now, but anyone who has seen him in action (including rottie, pit bull and Doberman owners) is in awe. He is very methodical and relentless - the mad genius of dog toy torture! Small and quiet but utterly ruthless. Anyway...my boy has been working on this since the beginning of October and the thing looks like new! I think you can see one tooth mark, otherwise it looks untouched! This is a dog who can reduce Kongs and other rubber toys to a pile of chunks in minutes. But he also LOVES it - carries it around, brings it into his bed at night to sleep, and when his doxie brother (who is far too dignified to do dog toys) doesn't feel well, it is offered up as a "feel better" snuggly...for about 2 minutes. But it's the thought that counts, right? I don't know what makes this so attractive to my mini-Jaws and so long-lasting, but it works!
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Reviewed in the United States on February 3, 2017
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Verified Purchase
Michael & Challice R.
Omaha, US
★★★★★ 5
HEAVEY Duty!
Color: Blue/Medium, Size: Blue
Almost not destructible! My dogs chew through 99% of the toys we purchase, but never this one! The medium size is good for our Doberman with jaws of steel!
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Reviewed in the United States on May 4, 2026

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